EFEK ANTIINFLAMASI KOMBINASI ASTAXANTHIN DENGAN VITAMIN E TERHADAP JUMLAH TROMBOSIT (Studi Eksperimental pada Tikus Putih Jantan Galur Wistar yang Diinduksi Karagenan)
DOI:
https://doi.org/10.71282/jurmie.v2i12.1493Keywords:
Astaxanthin, Inflammation, Carrageenan, Platelets, Vitamin EAbstract
Background: Inflammation is a defense reaction involving cells, mediators, and blood vessels, including platelets, to eliminate causes of cell damage. Carrageenan is a cause of cell damage that induces platelets through the NF-κB pathway by TLR-4 signaling. The combination of astaxanthin and vitamin E has anti-inflamatory effects characterized by a decrease in platelet count by suppressing NF-κB, COX-2, and ROS activity. Objective: This study aims to determine the effect of the combination of astaxanthin and vitamin E on platelet counts in male Wistar rats induced by carrageenan. Method: This study was experimental with a post-test only control group design. A total of 30 rats were adapted for 7 days with the same feed and drink, then the rats were divided into a normal group (n=10), a sick group (n=10), and a treatment group (n=10). The treatment group was given a combination of 0.72 mg/kgBW astaxanthin and 24.1 mg/kgBW vitamin E. One hour later, the sick and treatment groups were given a 0.1 mL injection of 1% carrageenan and waited for 24 hours, while the normal group was only given standard feed. The platelet count was measured using a hematology analyzer. The data were analyzed using the Shapiro Wilk test, Levene Statistic, Kruskal Wallis, and followed by the Mann Whitney Post Hoc test. Result: The mean platelet count was 144.86 × 10³/µL in the normal group, 316.79 × 10³/µL in the sick group, and 214.07 × 10³/µL in the treatment group. The results of the normality test KP (p<0.05), KN and KS (p>0.05), homogeneity (p<0.05), Kruskal Wallis (p<0.05) were significantly different, and the Post Hoc Mann Whintney (p<0.05) showed significant differences between all groups. Conclusion: Data analysis revealed an anti-inflammatory effect of the combination of astaxanthin and vitamin E in carrageenan-induced rats.
Downloads
References
Ariastuti, N. L. P., Candra, G. N. H., & Swastini, D. A. (2024). Profil Faktor Risiko Penyakit Jantung Koroner pada Kelompok Dewasa Muda. Journal of Language and Health, 5(2), 787–794. https://doi.org/10.37287/JLH.V5I1.4046
Chang, M. X., Xiong, F., Pak, S., & Liang Ooi, S. (2020). Astaxanthin and its Effects in Inflammatory Responses and Inflammation-Associated Diseases: Recent Advances and Future Directions. Molecules, 25(22), 5342. https://doi.org/10.3390/MOLECULES25225342
da Cunha Germano, B. C., de Morais, L. C. C., Idalina Neta, F., Fernandes, A. C. L., Pinheiro, F. I., do Rego, A. C. M., Araújo Filho, I., de Azevedo, E. P., de Paiva Cavalcanti, J. R. L., Guzen, F. P., & Cobucci, R. N. (2023). Vitamin E and Its Molecular Effects in Experimental Models of Neurodegenerative Diseases. International Journal of Molecular Sciences, 24(13). https://doi.org/10.3390/IJMS241311191
Federer, W. T. (1956). Experimental Design: Theory and Application. Forest Science, 2(3), 186–186. https://doi.org/10.1093/FORESTSCIENCE/2.3.186
Hannoodee, S., & Nasuruddin, D. N. (2024). Acute Inflammatory Response. Nature, 206(4979), 20. https://doi.org/10.1038/206020a0
Kemenkes. (2018). Laporan Riskesdas 2018 Nasional.pdf. In Lembaga Penerbit Balitbangkes (p. hal 156).
Kojok, K., El-Kadiry, A. E. H., & Merhi, Y. (2019). Role of NF-κB in platelet function. International Journal of Molecular Sciences, 20(17), 1–21. https://doi.org/10.3390/ijms20174185
Kumar, V., Abbas, A. K., Aster, J. C., Deyrup, A. T., & Das, A. (2023). Robbins and Kumar Basic Pathology (11 edition). Elsevier.
Laja, R. S. P. (2021). Astaxanthin untuk Kesehatan Kardiovaskular. Jurnal Penelitian Perawat Profesional, 3(2), 243–252. https://doi.org/10.37287/jppp.v3i2.259
Lala, M. D. T., & Sari, D. R. (2023). Perbandingan Gambaran Histologis Lapisan Piramidalis Area Ca1 Hipokampus Mus Musculus yang Dipapar Radiasi Gelombang Elektromagnetik Telepon Seluler 3G dan 4G. Jurnal Penelitian Perawat Profesional, 5(3), 1279–1290. https://doi.org/10.37287/jppp.v5i3.1772
Lewis, E. D., Meydani, S. N., & Wu, D. (2020). Regulatory Role of Vitamin E in the Immune System and Inflammation. 71(4), 487–494. https://doi.org/10.1002/iub.1976.Regulatory
Liao, S., Omage, S. O., Börmel, L., Kluge, S., Schubert, M., Wallert, M., & Lorkowski, S. (2022). Vitamin E and Metabolic Health: Relevance of Interactions with Other Micronutrients. Antioxidants, 11(9), 1785. https://doi.org/10.3390/ANTIOX11091785
Liu, J., Han, X., Zhang, T., Tian, K., Li, Z., & Luo, F. (2023). Reactive oxygen species (ROS) scavenging biomaterials for anti-inflammatory diseases: from mechanism to therapy. Journal of Hematology and Oncology, 16(1), 1–34. https://doi.org/10.1186/s13045-023-01512-7
Lopes, A. H., Silva, R. L., Fonseca, M. D., Gomes, F. I., Maganin, A. G., Ribeiro, L. S., Marques, L. M. M., Cunha, F. Q., Alves-Filho, J. C., Zamboni, D. S., Lopes, N. P., Franklin, B. S., Gombault, A., Ramalho, F. S., Quesniaux, V. F. J., Couillin, I., Ryffel, B., & Cunha, T. M. (2020). Molecular basis of carrageenan-induced cytokines production in macrophages. Cell Communication and Signaling, 18(1), 1–17. https://doi.org/10.1186/s12964-020-00621-x
Nair, A., Ahirwar, A., Singh, S., Lodhi, R., Lodhi, A., Rai, A., Jadhav, D. A., Harish, D. A., Varjani, S., Singh, G., Marchand, J., Schoefs, B., & Vinayak, V. (2023). Astaxanthin as a King of Ketocarotenoids: Structure, Synthesis, Accumulation, Bioavailability and Antioxidant Properties. Marine Drugs 2023, Vol. 21, Page 176, 21(3), 176. https://doi.org/10.3390/MD21030176
Pereira, C. P. M., Souza, A. C. R., Vasconcelos, A. R., Prado, P. S., & Name, J. J. (2020). Antioxidant and Anti‑Inflammatory Mechanisms of Action of Astaxanthin in Cardiovascular Diseases (Review).
Putri, S. Y., Isadiartuti, D., Isnaeni, Fahreza, A. F., Putri, A. V. M., Diana, Z., Fatihah, N. N., Arimbawa, I. G. R., Fakhirah, A., Wijayanata, T. N., Pangestu, M. P., Firdanthi, A., Addriana, O., & Rohma, U. A. (2024). The Effectiveness of Vitamin E Soft Capsules as an Antioxidant. Berkala Ilmiah Kimia Farmasi, 11(1), 5–11. https://doi.org/10.20473/bikfar.v11i1.51902
Rishel, R. A., & Basyir, V. (2018). Pengaruh Pemberian Vitamin E Terhadap Kadar Prostaglandin (PGF2A) dan TNF A pada Penderita Dismenorea. 9(2), 57–64.
Tao, Q., Fan, L. P., Feng, J., Zhang, Z. J., Liu, X. W., Qin, Z., Li, J. Y., & Yang, Y. J. (2024). Platelet Proteomics and Tissue Metabolomics Investigation for the Mechanism of Aspirin Eugenol Ester on Preventive Thrombosis Mechanism in a Rat Thrombosis Model. International Journal of Molecular Sciences, 25(19), 10747. https://doi.org/10.3390/IJMS251910747/S1
Widyarini, S., Sugiyono, Akrom, A. M., & Paryuni, A. D. (2023). Carrageenan-Induced Acute Inflammation on Back-Skin of Mice: Histopathological Features, Number of Inflammatory Cells, and Expression of COX-2, COX-1, and IL-6. World’s Veterinary Journal, 13(4), 520–530. https://doi.org/10.54203/SCIL.2023.WVJ55
Wu, Q., Luo, Y., Lu, H., Xie, T., Hu, Z., Chu, Z., & Luo, F. (2024). The Potential Role of Vitamin E and the Mechanism in the Prevention and Treatment of Inflammatory Bowel Disease. Foods 2024, Vol. 13, Page 898, 13(6), 898. https://doi.org/10.3390/FOODS13060898
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Salsabila Tria Ramadhani, Sampurna (Author)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
