PENGARUH PEMBERIAN EKSTRAK ETANOL BUNGA TELANG (Clitoria ternatea) TERHADAP KADAR IL-10 (INTERLEUKIN 10) (Studi Ekperimental pada Tikus putih strain Wistar jantan yang dipapar Asap Rokok)

Abstract

Cigarette smoke exposure contains toxic substances such as nicotine and various harmful compounds that can induce oxidative stress and disrupt immune regulation. This condition stimulates inflammatory and anti-inflammatory responses, including changes in Interleukin-10 (IL-10) levels. Butterfly pea flower (Clitoria ternatea L.) contains flavonoids and anthocyanins with antioxidant and anti-inflammatory properties that may modulate inflammatory responses caused by cigarette smoke exposure. This study aimed to determine the effect of ethanol extract of butterfly pea flower on IL-10 levels in male Wistar rats exposed to cigarette smoke. This was an experimental study using a post-test only control group design involving 30 male Wistar rats divided into five groups. Treatments were administered for 14 days. On day 15, blood samples were collected and IL-10 levels were measured using ELISA. Data were analyzed using One-Way ANOVA followed by Post-Hoc LSD test. The mean IL-10 levels were 0.350 ng/mL (normal control), 0.818 ng/mL (negative control), 0.566 ng/mL (aspirin), 0.806 ng/mL (200 mg/200gBW), and 0.961 ng/mL (400 mg/200gBW). One-Way ANOVA showed significant differences among groups (p < 0.05). The results showed significant differences among groups (p < 0.05). It can be concluded that ethanol extract of Clitoria ternatea affects IL-10 levels in male Wistar rats exposed to cigarette smoke.